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Part:BBa_K1590003:Design

Designed by: Manuel Blank   Group: iGEM15_Dundee   (2015-09-09)


Chromate responsive promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 135
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter was excised as a separate part in order to build a chromate-inducible fluorescent reporter.

Primers for amplification of pChr from BBa_K1058008. 

  Forward: GCGC GAATTCGCGGCCGCTTCTAGAG ATTGCTTATTCCTATTGC

  Reverse: GCGC CTGCAGCGGCCGCTACTAGT AGTCGTAGATGTTACTACA



Source

PCR-amplification from biobrick BBa_K1058008 (pChrBGFP).

References